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Disease models

Collection of haploid human isogeneic cell lines with mutations in individual genes

A collection of haploid human isogeneic cell lines with single mutations could be useful for creating new disease models and identifying therapeutic targets. The collection was generated by mutating and barcoding 3,396 genes in stable haploid human cells cloned from a chronic myelogenous leukemia cell line. The mutagenesis method induced reversible mutations in the haploid cells that cause near complete inactivation of the targeted gene. Next steps include increasing the number of knockout cell lines to cover additional genes and using the cell lines to support target and drug discovery efforts at Haplogen GmbH (see Trapping human genes, page 8).

SciBX 6(37); doi:10.1038/scibx.2013.1043
Published online Sept. 26, 2013

Patent status undisclosed; available for licensing and partnerships through Haplogen

Bürckstümmer, T. et al. Nat. Methods; published online Aug. 25, 2013;
doi:10.1038/nmeth.2609
Contact: Sebastian M.B. Nijman, Research Center for Molecular Medicine of the Austrian Academy of Sciences, Vienna, Austria
e-mail:
snijman@cemm.oeaw.ac.at