This week in techniques



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Assays & screens

Highly specific, recombinant antibodies to detect histone H3 trimethylation

Antibodies highly specific for H3K4me3 and H3K9me3 could be used to aid epigenetic drug development. A problem with the analysis of histone modifications is that many antibodies lack lot-to-lot consistency and may be nonspecific. Phage display and in vitro mutagenesis were used to generate recombinant antibodies with greater specificity for H3K4me3 or H3K9me3 than commercially available antibodies. In vitro, the H3K9me3 antibody was used in a histone methyltransferase assay to generate a stronger detection signal than was seen when using a commercially available antibody. Next steps include establishing drug screening and diagnostic assays using the antibodies.

SciBX 6(37); doi:10.1038/scibx.2013.1038
Published online Sept. 26, 2013

Patent application filed; available for licensing

Hattori, T. et al. Nat. Methods; published online Aug.18, 2013;
Contact: Shohei Koide, The University of Chicago, Chicago, Ill.