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Design of motif-specific antibody scaffolds targeting post-translational modifications

Motif-specific antibody scaffolds for use in phage display libraries could help generate mAbs that target post-translational modifications. Current methods to generate mAbs that target post-translational modifications largely rely on in vivo immunization, which is generally lower throughput, slower and less precise than the generation of mAbs via in vitro methods. mAb scaffolds were designed with pockets that bind to phosphorylated serine, threonine or tyrosine and were used to generate two single-chain Fv mAb phage display libraries. The libraries yielded 51 mAbs that bound to 7 different peptides containing phosphorylated amino acid residues with affinities ranging from 42 to 5,000 nM. Next steps include automating the process and designing mAb scaffolds against other post-translational modifications.

SciBX 6(35); doi:10.1038/scibx.2013.976
Published online Sept. 12, 2013

Provisional patent application filed; available for licensing

Koerber, J.T. et al. Nat. Biotechnol.; published online Aug. 18, 2013;
Contact: James A. Wells, University of California, San Francisco, Calif.