This week in techniques



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Assays & screens

Phenotypic neuronal assays to screen for small molecules that decrease b-amyloid (Ab)

Phenotypic screening of embryonic stem cell (ESC)-derived neurons could help identify small molecules that decrease Ab to treat Alzheimer's disease (AD). The assays used ESC-derived neurons generated from the Tg2576 mouse model of AD and allowed the monitoring of the synthesis and synaptic activity of Ab. The assay was used to screen a library of 446 small molecules and yielded 4 hits that inhibited Ab synthesis with single-digit micromolar and submicromolar IC50 values. Next steps include integrating the assay into an automated high throughput screening platform that operates in a 384-well format.

SciBX 6(33); doi:10.1038/scibx.2013.904
Published online Aug. 29, 2013

Patent application filed; available for licensing from Columbia Technology Ventures

McIntire, L.B.J. et al. Chem. Biol.; published online July 25, 2013;
Contact: Tae-Wan Kim, Columbia University Medical Center, New York, N.Y.