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Genetically encoded fluorescent calcium sensors for neuronal activity imaging

In vitro and mouse studies identified fluorescent calcium sensors that could improve the sensitivity of neuronal imaging. Mutagenesis studies identified optimized, genetically encoded fluorescent calcium sensors with better sensitivity and kinetics than other synthetic or genetically encoded sensors. In mice, adenovirus vector-encoded optimized sensors enabled reliable reporting of both single action potentials and activity in large groups of neurons for up to two months without perturbing cortical circuits. Next steps include using the sensors as research tools.

SciBX 6(32); doi:10.1038/scibx.2013.878
Published online Aug. 22, 2013

Patent application filed; available for nonexclusive licensing to not-for-profit researchers and commercial entities

Chen, T.-W. et al. Nature; published online July 17, 2013;
doi:10.1038/nature12354
Contact: Douglas S. Kim, Howard Hughes Medical Institute, Ashburn, Va.
e-mail:
kimd@janelia.hhmi.org

Contact: Karel Svoboda, same affiliation as above
e-mail:
svobodak@janelia.hhmi.org

Contact: Loren L. Looger, same affiliation as above
e-mail:
loogerl@janelia.hhmi.org

Contact: Vivek Jayaraman, same affiliation as above
e-mail:
jayaramanv@janelia.hhmi.org

Contact: Michael B. Orger, Champalimaud Centre for the Unknown, Lisbon, Portugal
e-mail:
michael.orger@neuro.fchampalimaud.org