Thursday, August 15, 2013
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Assays & screens
enrichment to identify broadly neutralizing influenza A antibodies with high
A strategy to enrich human
plasmablasts could be used to identify broadly neutralizing influenza A
antibodies with high frequency. Peripheral blood mononuclear cells (PBMCs)
from human donors vaccinated against influenza A virus were treated with an influenza A virus hemagglutinin
antigen premix and transplanted into the spleens of immunodeficient mice to
allow for rapid expansion and differentiation into human plasmablasts.
Isolated plasmablasts bound influenza A virus variants with 150-fold higher
frequency than did plasmablasts derived from untreated PBMCs. In 950 enriched
plasmablasts, two broadly neutralizing mAbs were identified through the
screening of only 840 cloned antibodies, whereas the previously described,
broadly neutralizing mAb FI6 was identified through
analysis of about 104,000 cultured human plasma cells. Next steps could
include testing the antibodies in humans.
FI6 is being developed by Humabs BioMed S.A., which
has licensed the product to an undisclosed pharma.
Published online Aug. 15, 2013
Patent and licensing status
Nakamura, G. et al. Cell
Host Microbe; published online July 17, 2013;
Contact: Lee R. Swem, Genentech, South San Francisco, Calif.
Contact: Mercedesz Balazs, same affiliation as above
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