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Disease models

Organoid model for colon cancer derived from primary intestinal epithelial cells

Primary intestinal epithelial cells modified to form tumorigenic organoids in 3D cell culture could provide an in vitro model for colon cancer. In 3D cultures of primary intestinal epithelial cells, lentiviral-mediated small hairpin RNA knockdown of adenomatous polyposis coli (APC) led to the production of organoids with genetic features comparable to those in APC-deficient adenomas. In nude mice, injection of APC-deficient organoids led to intestinal cancer-like tumors with cancer stem cell-like properties. Next steps include testing knockdown of additional colon cancer genes in the model.

SciBX 6(28); doi:10.1038/scibx.2013.737
Published online July 25, 2013

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Onuma, K. et al. Proc. Natl. Acad. Sci. USA; published online June 17, 2013;
Contact: Yoshitaka Hippo, National Cancer Center Research Institute, Tokyo, Japan