Thursday, July 25, 2013
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Organoid model for colon
cancer derived from primary intestinal epithelial cells
epithelial cells modified to form tumorigenic organoids in 3D cell culture
could provide an in vitro model for colon cancer. In 3D cultures of
primary intestinal epithelial cells, lentiviral-mediated small hairpin RNA
knockdown of adenomatous
polyposis coli (APC)
led to the production of organoids with genetic features comparable to those
in APC-deficient adenomas. In nude mice, injection of APC-deficient
organoids led to intestinal cancer-like tumors with cancer stem cell-like
properties. Next steps include testing knockdown of additional colon cancer
genes in the model.
Published online July 25, 2013
status not applicable
Onuma, K. et al. Proc.
Natl. Acad. Sci. USA; published online June 17, 2013;
Contact: Yoshitaka Hippo, National Cancer Center Research
Institute, Tokyo, Japan
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