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Phage display system for rapid mammalian expression of library hits

A phage display system designed to facilitate expression in mammalian cells could be used to rapidly screen libraries against therapeutic targets. A time-consuming step in phage display is the cloning of library candidates into a mammalian expression system. An expression vector was designed to take advantage of splicing machinery unique to mammalian cells. One version of a candidate fusion protein is produced in bacterial cells, and another, distinct version of the fusion protein is produced in mammalian cells. The system was used to discover a CD4-mimetic peptide that binds to HIV-1 envelope glycoprotein and neutralizes the virus. Next steps include modifying the expression vector to enable rapid antibody selection and expression.

SciBX 6(23); doi:10.1038/scibx.2013.587
Published online June 13, 2013

Provisional patent application filed; available for licensing from Harvard University Office of Technology Development

Quinlan, B.D. et al. J. Biol. Chem.; published online May 10, 2013;
doi:10.1074/jbc.M113.452839
Contact: Michael Farzan, The Scripps Research Institute, Jupiter, Fla.
e-mail:
mfarzan@scripps.edu

Contact: Brian Quinlan, same affiliation as above
e-mail:
bquinlan@scripps.edu