Thursday, May 23, 2013
Publication and contact
Assays & screens
Fluorescence labels in
phosphatase (FLiP) assay to detect allosteric inhibitors of phosphatases
A FLiP assay could help
identify allosteric inhibitors of phosphatases. An allosteric inhibitor of protein
tyrosine phosphatase 1B (PTP-1B;
bound a pocket about 20 Å from the enzyme's active site and blocked
phosphatase activity by displacing the a7 helix from the
phosphatase core. A high throughput FLiP assay was created by labeling the
PTP-1B a7 helix with a red fluorophore, which produced
altered emission spectra when displaced. In this assay, the allosteric
inhibitor shifted the emission spectra readout, whereas an active site-targeting
inhibitor or nonbinding control molecule did not. Next steps could include
using the screen to identify additional phosphatase inhibitors.
SciBX 6(20); doi:10.1038/scibx.2013.498
Published online May 23, 2013
Patent and licensing status
Schneider, R. et al. J.
Am. Chem. Soc.; published online April 23, 2013;
Contact: Daniel Rauh, Technical University of Dortmund, Dortmund,
All contents Copyright © 1993-2016 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]