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Using integrin a2 (VLA-2; CD49B) and lymphocyte-activation gene 3 (LAG3; CD223) to isolate type 1 Treg cells

CD49B and LAG3 could be useful for identifying and isolating populations of type 1 Treg cells, which promote and maintain immune tolerance. In both mouse and human type 1 Treg cells, expression profiling showed that CD49B and LAG3 were stably and selectively coexpressed on the cell surface. Fluorescence-activated cell sorting (FACS) isolated T cell populations enriched for CD49B+ and LAG3+ cells. In culture, the enriched T cell population had greater immunosuppressive capacity and IL-10 secretion than the original, unenriched T cell population. Next steps include comparing the activity of type 1 Treg cells from healthy subjects and patients who have autoimmune diseases and developing clinical-grade protocols to isolate and purify such cells.

SciBX 6(19); doi:10.1038/scibx.2013.477
Published online May 16, 2013

Provisional patent application filed; available for licensing from the San Raffaele Scientific Institute Office of Biotechnology Transfer
Contact: Paola Pozzi, San Raffaele Scientific Institute, Milan, Italy

Gagliani, N. et al. Nat. Med.; published online April 28, 2013;
Contact: Maria-Grazia Roncarolo, San Raffaele Scientific Institute, Milan, Italy
Contact: Richard A. Flavell,
Yale University, New Haven, Conn.