Thursday, May 16, 2013
This week in techniques
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Using integrin a2 (VLA-2;
gene 3 (LAG3;
to isolate type 1 Treg cells
CD49B and LAG3 could be
useful for identifying and isolating populations of type 1 Treg
cells, which promote and maintain immune tolerance. In both mouse and human
type 1 Treg cells, expression profiling showed that CD49B and LAG3
were stably and selectively coexpressed on the cell surface.
Fluorescence-activated cell sorting (FACS) isolated T cell populations
enriched for CD49B+ and LAG3+ cells. In
culture, the enriched T cell population had greater immunosuppressive
capacity and IL-10 secretion than the original,
unenriched T cell population. Next steps include comparing the activity of
type 1 Treg cells from healthy subjects and patients who have
autoimmune diseases and developing clinical-grade protocols to isolate and
purify such cells.
Published online May 16, 2013
application filed; available for licensing from the San
Raffaele Scientific Institute Office of Biotechnology
Contact: Paola Pozzi, San Raffaele Scientific Institute, Milan,
Gagliani, N. et al. Nat.
Med.; published online April 28, 2013;
Contact: Maria-Grazia Roncarolo, San Raffaele Scientific
Institute, Milan, Italy
Contact: Richard A. Flavell,
Yale University, New Haven, Conn.
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