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Assays & screens

Yeast chemical genetic screen for inhibitors of human telomerase

A yeast chemical genetic screening assay for small molecule inhibitors of human telomerase could help identify new leads to treat telomerase-positive cancers. The assay identified small molecules that could restore growth in an engineered strain of yeast that undergoes inducible growth arrest in the presence of human telomerase. In a library of 678 compounds, the screening assay identified 8 such molecules. In a series of in vitro assays, three of the eight identified molecules were shown to inhibit human telomerase. Next steps could include testing the molecules in telomerase-positive cancer cell lines and screening a larger compound library in the yeast-based assay.

SciBX 6(14); doi:10.1038/scibx.2013.346
Published online April 11, 2013

Patent and licensing status undisclosed

Wong, L.H. et al. Chem. Biol.; published online March 21, 2013;
doi:10.1016/j.chembiol.2012.12.008
Contact: Lea Harrington, University of Montreal, Montreal, Quebec, Canada
e-mail:
lea.harrington@umontreal.ca