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Sequence-specific labeling of multiple cell types in a sample using surface zinc fingers (sZFs) and fluorophore-tagged DNA probes

In vitro studies suggest a method using sZFs and fluorophore-tagged DNA probes could be useful for imaging multiple cell types in complex tissue samples. Cells were labeled with cell surface-binding zinc fingers and then fluorescently tagged with fluorophore probes containing double-stranded DNA specific for a particular sZF. In a mixed population of cells labeled with different sZFs, addition of fluorophore-tagged probes specific for the different sZFs enabled the imaging of individual cell populations as distinct fluorescent signals. Next steps could include using the method to image cells in human tissue samples.

SciBX 6(13); doi:10.1038/scibx.2013.324
Published online April 4, 2013

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Mali, P. et al. Nat. Methods; published online March 17, 2013;
Contact: George M. Church, Harvard Medical School, Boston, Mass.