Thursday, March 14, 2013
Publication and contact
Assays & screens
MicroRNA detection with
hairpin-mediated quadratic enzymatic amplification (HQEA)
HQEA for miRNAs could
enable sensitive detection of disease-associated miRNAs in clinical samples.
The method involves hybridizing a molecular beacon probe with the targeted
miRNA and then synthesizing an inactive DNA duplex of the probe that is
activated by an endonuclease to emit the fluorescent signal. In a
proof-of-concept study, a detection assay using HQEA detected miRNA-21
in a 15 microliter sample with a detection limit of
10 femtomolar at 37 °C and 1 attomolar at 4 °C. In cell lysates from patient
breast cancer samples or human breast and prostate cancer cell lines, the
assay detected miR-21 and miR-221. Next steps include developing
gene chips that use HQEA for detecting disease-associated miRNAs.
Published online March 14, 2013
Patented; unavailable for
Duan, R. et al. J. Am.
Chem. Soc.; published online
Feb. 27, 2013;
Contact: Fan Xia, Huazhong University of Science &
Technology, Wuhan, China
All contents Copyright © 1993-2016 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]