Thursday, February 28, 2013
Publication and contact
Comprehensive library of
human genome-targeted transcription activator-like effector nucleases
A library of TALENs
designed to disrupt every protein-coding region of the human genome could be
used to generate new disease models. A computational algorithm was developed
to identify unique 40-base-pair sites in the 5ʹ end of
protein-coding genes that have minimal potential for off-target effects.
Using this information, about 18,000 gene-targeting TALEN pairs were cloned
and assembled in 96-well plates. In cultured cells, a test set of 126 TALENs
induced site-specific mutations with a 98.4% success rate. Next steps include
further optimizing the TALENs and using this approach to perform functional
Published online Feb. 28, 2013
Patented; TALEN plasmids
available from Seoul
National University through its TALEN
library; TALEN-modified cells available for purchase from ToolGen=
Kim, Y. et al. Nat.
Biotechnol.; published online Feb. 17, 2013;
Contact: Jin-Soo Kim, Seoul National University, Seoul,
Contact: Seokjoong Kim, ToolGen Inc., Seoul, South Korea
All contents Copyright © 1993-2017 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]