Thursday, February 28, 2013
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Comprehensive library of
human genome-targeted transcription activator-like effector nucleases
A library of TALENs
designed to disrupt every protein-coding region of the human genome could be
used to generate new disease models. A computational algorithm was developed
to identify unique 40-base-pair sites in the 5ʹ end of
protein-coding genes that have minimal potential for off-target effects.
Using this information, about 18,000 gene-targeting TALEN pairs were cloned
and assembled in 96-well plates. In cultured cells, a test set of 126 TALENs
induced site-specific mutations with a 98.4% success rate. Next steps include
further optimizing the TALENs and using this approach to perform functional
Published online Feb. 28, 2013
Patented; TALEN plasmids
available from Seoul
National University through its TALEN
library; TALEN-modified cells available for purchase from ToolGen=
Kim, Y. et al. Nat.
Biotechnol.; published online Feb. 17, 2013;
Contact: Jin-Soo Kim, Seoul National University, Seoul,
Contact: Seokjoong Kim, ToolGen Inc., Seoul, South Korea
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