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Disease models

Comprehensive library of human genome-targeted transcription activator-like effector nucleases (TALENs)

A library of TALENs designed to disrupt every protein-coding region of the human genome could be used to generate new disease models. A computational algorithm was developed to identify unique 40-base-pair sites in the 5ʹ end of protein-coding genes that have minimal potential for off-target effects. Using this information, about 18,000 gene-targeting TALEN pairs were cloned and assembled in 96-well plates. In cultured cells, a test set of 126 TALENs induced site-specific mutations with a 98.4% success rate. Next steps include further optimizing the TALENs and using this approach to perform functional knockout studies.

SciBX 6(8); doi:10.1038/scibx.2013.202
Published online Feb. 28, 2013

Patented; TALEN plasmids available from Seoul National University through its TALEN library; TALEN-modified cells available for purchase from ToolGen= Inc.

Kim, Y. et al. Nat. Biotechnol.; published online Feb. 17, 2013;
Contact: Jin-Soo Kim, Seoul National University, Seoul, South Korea

Contact: Seokjoong Kim, ToolGen Inc., Seoul, South Korea