Thursday, February 14, 2013
Publication and contact
Assays & screens
through an a-hemolysin-caseinolytic peptidase X homolog (ClpX)
A method to translocate
proteins through a-hemolysin nanopores could eventually enable
nanopore-based protein sequencing. There are nanopore-based DNA sequencing
methods in commercial development that detect and identify DNA by measuring
voltage changes as individual base pairs pass through a membrane-embedded
nanopore, but these methods cannot identify peptides. To enable protein
translocation through a membrane-embedded nanopore, the AAA+ unfoldase ClpX was added in solution to one side of
the membrane, allowing detection of structure-dependent voltage changes as a
protein substrate of about 100 amino acids and carrying a ClpX binding tag
passed through the pore. Next steps could include determining the relationship
between voltage change and amino acid identity.
At least five companies are developing nanopore-based DNA sequencing systems.
Published online Feb. 14, 2013
Patent and licensing status
Nivala, J. et al. Nat.
Biotechnol.; published online Feb. 3, 2013;
Contact: Mark Akeson, University of California, Santa Cruz, Calif.
All contents Copyright © 1993-2016 BioCentury Publications, Inc. ALL RIGHTS RESERVED. All use of this Web Site and its contents is governed by the BioCentury User Agreement
. The contents of this Web Site are protected under U.S. and foreign copyright and intellectual property laws, and no part of this Web Site or its contents may be photocopied, reproduced or retransmitted in any form without the written consent of BioCentury, which may be requested from Reprints/Permissions
. BioCentury®; The Bernstein Report on BioBusiness™; The BioCentury 100™; The Clear Route to ROI™; Because Real Intelligence is Hard to Find™; BCIQ™; and BioPharma's Knowledge Center™; are trademarks of BioCentury Publications, Inc., P.O. Box 1246, San Carlos, CA 94070. SciBX® and SciBX: Science-Business eXchange® are trademarks of Nature America, Inc. that are jointly used by BioCentury Publications, Inc. and Nature America, Inc.
Thanks for purchasing this article.
Please bookmark this url for future reference.
We have also sent you a confirming e-mail with a receipt and this same url for your
close [ X ]