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Assays & screens

Adapted Plasmodium knowlesi parasites that infect cultured human red blood cells

Culture of modified P. knowlesi strains could help identify new malaria therapies. Wild-type P. knowlesi survive and replicate in macaque but not human red blood cells. The parasites were adapted to infect human red blood cells by culturing them in a 4:1 mixture of human and macaque cells. After eight months of continuous coculture, the adapted parasites infected and replicated in cultured human red blood cells. Next steps include using the adapted parasite to screen for therapeutics and vaccines against P. knowlesi and related parasites.

SciBX 6(3); doi:10.1038/scibx.2013.65
Published online Jan. 24, 2013

Unpatented; licensing status not applicable

Moon, R.W. et al. Proc. Natl. Acad. Sci. USA; published online Dec. 24, 2012;
Contact: Michael J. Blackman, Medical Research Council National Institute for Medical Research, London, U.K.
Contact: Anthony A. Holder, same affiliation as above
Contact: Robert W. Moon, same affiliation as above