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Assays & screens

Transforming growth factor-a (TGFA; TGFa) shedding assay to detect G protein-coupled receptor (GPR) ligands

A TGFa shedding assay could help identify GPR ligands. TGFa shedding occurs downstream of GPR activation when certain Ga subunits are activated. In cell culture, the TGFa shedding assay detected receptor activity for 104 out of 116 GPRs. In cells expressing histamine receptor subtypes, only ligands that selectively activated a particular receptor subtype induced shedding. Next steps include modifying the platform to involve fewer steps.

SciBX 5(39); doi:10.1038/scibx.2012.1039
Published online Oct. 4, 2012

Findings unpatented; unavailable for licensing

Inoue, A. et al. Nat. Methods; published online Sept. 16, 2012;
Contact: Junken Aoki, Tohoku University, Sendai, Japan