Thursday, October 4, 2012
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Assays & screens
receptor-capturing (LRC) technology to identify cell surface receptors of
therapeutic or pathologic ligands
LRC technology could be
used to identify cell surface receptor targets of ligands. The technology
uses a chemoreactive reagent that contains a glycosylated cell surface-binding
moiety, a biotin tag and an amino-reactive moiety, which binds to a ligand of
interest. In cell culture or tissue, the ligand-bound chemoreactive agent
attached to the ligand's cell surface receptor and the biotin tag was used to
purify the receptor for identification using mass spectroscopy. In various
cells, receptors for ligands, including insulin
and transferrin, were
identified. In cell culture, the LRC technology identified five
surface-binding proteins for vaccinia virus, which were verified using small
interfering RNA-mediated knockout of the receptors. Next steps include using
the platform for drug development.
Published online Oct. 4, 2012
Patent application filed;
available for licensing
Frei, A.P. et al. Nat.
Biotechnol.; published online Sept. 16, 2012;
Contact: Bernd Wollscheid, Swiss Federal Institute of
Technology Zurich (ETHZ), Zurich, Switzerland
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