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Tools & Techniques
Making introns useful
Monday, July 24, 2000
Introns are generally regarded as portions of messenger RNA that do not
contain protein-coding information and are discarded after RNA splicing. But certain
intronic RNA species have activities that could be useful for gene therapy or functional
genomics. Whereas viral gene therapy vectors have yet to overcome immunogenicity and
efficiency problems and often integrate at random sites in the genome, researchers are
beginning to explore alternative methods for disrupting genes in a specific, stable manner
in a wide range of cell types
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